NTISthis.com

Evidence Guide: MSL974006 - Perform biological procedures

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL974006 - Perform biological procedures

What evidence can you provide to prove your understanding of each of the following citeria?

Interpret and schedule work requirements

  1. Review work request to identify samples, required procedures and materials/equipment/instruments involved
  2. Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and/or equipment
  3. Plan parallel work sequences to optimise throughput of multiple sets of samples
Review work request to identify samples, required procedures and materials/equipment/instruments involved

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and/or equipment

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Plan parallel work sequences to optimise throughput of multiple sets of samples

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Receive and prepare biological samples

  1. Log samples using standard operating procedures (SOPs)
  2. Record sample description, compare with specification and note and report discrepancies
  3. Prepare samples in accordance with testing requirements
  4. Ensure traceability of sample from receipt to reporting of results
Log samples using standard operating procedures (SOPs)

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Record sample description, compare with specification and note and report discrepancies

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare samples in accordance with testing requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure traceability of sample from receipt to reporting of results

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform techniques that assist in the classification of a cell or species

  1. Select suitable techniques in accordance with workplace requirements and methods
  2. Set up and use equipment and reagents in accordance with the method
  3. Perform techniques in accordance with the method
Select suitable techniques in accordance with workplace requirements and methods

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Set up and use equipment and reagents in accordance with the method

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform techniques in accordance with the method

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform techniques that analyse biological activity

  1. Select suitable techniques in accordance with workplace requirements and methods
  2. Set up and use equipment and reagents in accordance with the method
  3. Perform techniques in accordance with the method
Select suitable techniques in accordance with workplace requirements and methods

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Set up and use equipment and reagents in accordance with the method

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform techniques in accordance with the method

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Process and interpret data

  1. Record test data noting atypical observations
  2. Construct calibration graphs and compute results for all samples from these graphs
  3. Ensure calculated values are consistent with expectations
  4. Record and report results in accordance with workplace procedures
  5. Estimate and document uncertainty of measurement in accordance with workplace procedures
  6. Interpret trends in data and/or results and report out-of-specification or atypical results promptly to appropriate personnel
  7. Determine if obvious procedure or equipment problems have led to atypical data or results
Record test data noting atypical observations

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Construct calibration graphs and compute results for all samples from these graphs

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure calculated values are consistent with expectations

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Record and report results in accordance with workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Estimate and document uncertainty of measurement in accordance with workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Interpret trends in data and/or results and report out-of-specification or atypical results promptly to appropriate personnel

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Determine if obvious procedure or equipment problems have led to atypical data or results

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a safe work environment

  1. Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel
  2. Minimise the generation of waste
  3. Ensure the safe disposal of biohazardous wastes
  4. Clean, care for and store equipment and reagents as required
Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Minimise the generation of waste

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure the safe disposal of biohazardous wastes

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Clean, care for and store equipment and reagents as required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain laboratory records

  1. Record approved data into workplace system
  2. Maintain confidentiality and security of workplace information and laboratory data
  3. Maintain equipment and calibration logs in accordance with workplace procedures
Record approved data into workplace system

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain confidentiality and security of workplace information and laboratory data

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain equipment and calibration logs in accordance with workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Evidence Guide

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1

Interpret and schedule work requirements

1.1

Review work request to identify samples, required procedures and materials/equipment/instruments involved

1.2

Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and/or equipment

1.3

Plan parallel work sequences to optimise throughput of multiple sets of samples

2

Receive and prepare biological samples

2.1

Log samples using standard operating procedures (SOPs)

2.2

Record sample description, compare with specification and note and report discrepancies

2.3

Prepare samples in accordance with testing requirements

2.4

Ensure traceability of sample from receipt to reporting of results

3

Perform techniques that assist in the classification of a cell or species

3.1

Select suitable techniques in accordance with workplace requirements and methods

3.2

Set up and use equipment and reagents in accordance with the method

3.3

Perform techniques in accordance with the method

4

Perform techniques that analyse biological activity

4.1

Select suitable techniques in accordance with workplace requirements and methods

4.2

Set up and use equipment and reagents in accordance with the method

4.3

Perform techniques in accordance with the method

5

Process and interpret data

5.1

Record test data noting atypical observations

5.2

Construct calibration graphs and compute results for all samples from these graphs

5.3

Ensure calculated values are consistent with expectations

5.4

Record and report results in accordance with workplace procedures

5.5

Estimate and document uncertainty of measurement in accordance with workplace procedures

5.6

Interpret trends in data and/or results and report out-of-specification or atypical results promptly to appropriate personnel

5.7

Determine if obvious procedure or equipment problems have led to atypical data or results

6

Maintain a safe work environment

6.1

Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel

6.2

Minimise the generation of waste

6.3

Ensure the safe disposal of biohazardous wastes

6.4

Clean, care for and store equipment and reagents as required

7

Maintain laboratory records

7.1

Record approved data into workplace system

7.2

Maintain confidentiality and security of workplace information and laboratory data

7.3

Maintain equipment and calibration logs in accordance with workplace procedures

Required Skills and Knowledge

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1

Interpret and schedule work requirements

1.1

Review work request to identify samples, required procedures and materials/equipment/instruments involved

1.2

Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and/or equipment

1.3

Plan parallel work sequences to optimise throughput of multiple sets of samples

2

Receive and prepare biological samples

2.1

Log samples using standard operating procedures (SOPs)

2.2

Record sample description, compare with specification and note and report discrepancies

2.3

Prepare samples in accordance with testing requirements

2.4

Ensure traceability of sample from receipt to reporting of results

3

Perform techniques that assist in the classification of a cell or species

3.1

Select suitable techniques in accordance with workplace requirements and methods

3.2

Set up and use equipment and reagents in accordance with the method

3.3

Perform techniques in accordance with the method

4

Perform techniques that analyse biological activity

4.1

Select suitable techniques in accordance with workplace requirements and methods

4.2

Set up and use equipment and reagents in accordance with the method

4.3

Perform techniques in accordance with the method

5

Process and interpret data

5.1

Record test data noting atypical observations

5.2

Construct calibration graphs and compute results for all samples from these graphs

5.3

Ensure calculated values are consistent with expectations

5.4

Record and report results in accordance with workplace procedures

5.5

Estimate and document uncertainty of measurement in accordance with workplace procedures

5.6

Interpret trends in data and/or results and report out-of-specification or atypical results promptly to appropriate personnel

5.7

Determine if obvious procedure or equipment problems have led to atypical data or results

6

Maintain a safe work environment

6.1

Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel

6.2

Minimise the generation of waste

6.3

Ensure the safe disposal of biohazardous wastes

6.4

Clean, care for and store equipment and reagents as required

7

Maintain laboratory records

7.1

Record approved data into workplace system

7.2

Maintain confidentiality and security of workplace information and laboratory data

7.3

Maintain equipment and calibration logs in accordance with workplace procedures

Evidence of competence in this unit must satisfy all of the requirements of the elements and performance criteria, and include demonstration of:

safely performing at least five (5) routine biological techniques that involve several steps to classify cell types, species and biologically active compounds by analysing their biological and chemical characteristics

accurately interpreting work requirements and following test procedures and safe work practices

safely preparing samples and using separation techniques

conducting pre-use and calibration checks on equipment

safely operating test equipment and instruments for qualitative and quantitative analysis in accordance with workplace procedures and manufacturer specifications

maintaining and evaluating reagents

processing data, estimating uncertainties, preparing calibration graphs and calculating results in appropriate units and precision

applying basic theoretical knowledge to interpret gross features of data and making relevant conclusions

troubleshooting obvious departures from standard procedures

identifying atypical results as out-of-normal range or an artefact using reference material or quality control sera

tracing and sourcing obvious causes of an artefact

communicating problems to a supervisor or outside service technician

recording and communicating results according to workplace procedures

maintaining security, integrity, traceability and identity of samples, sub-samples and documentation

safely collecting, storing and disposing of biohazardous wastes.

Must provide evidence that demonstrates knowledge of:

biological principles and concepts underpinning tests and procedures associated with job role, such as:

chemical and biochemical characteristics of lipids, carbohydrates, nucleic acids and proteins influencing structure, function and reactivity both in vitro and in vivo

molecular interactions within the compounds of nucleic acids and nucleotides, proteins and amino acids, carbohydrates, lipids and vitamins, influencing structure, activity, chemical reactivity and physical properties, including solubility, energy levels and emission/absorption spectra

chemical significance of biologically significant ions, such as calcium, zinc, iron, magnesium, sodium, potassium, chloride and phosphate

basic structure and function of organelles, cells, plant and animal tissue and organs relevant to the testing requirements

interrelationships of biological systems (carbon cycle, energy cycle, eutrophication and the web of life)

classification and taxonomy of organisms, such as bacteria, viruses, yeasts, single cell, multi-cellular, plants, animals, prions, helminths, prokaryotes and eukaryotes

phases of the cell cycle

Mendelian genetics, such as inheritance, meiosis, karyotypes, dominant and recessive traits, genotypes and phenotypes, and pedigrees

significance of the genetic code and transcription and translation

cell membrane activity, including diffusion (passive, facilitated and active), osmosis, tonicity and plasmolysis

staining reactions involving acid/base, redox, complex ion formation, solubility and equilibrium

operating principles and function of key components of the equipment and instruments used as part of job role

effects of modifying equipment and instrument variables

basic equipment and method troubleshooting procedures

calculation steps to give results in appropriate units and precision

sources of uncertainty in measurement and methods for control

importance and appropriate use of controls and certified reference materials

workplace and legal requirements for traceability

relevant hazards and risks in biological laboratories, work health and safety (WHS) and environmental requirements.

Range Statement

This field allows for different work environments and conditions that may affect performance. Essential operating conditions that may be present (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) are included.

Standards, codes, procedures and/or workplace requirements

Standards, codes, procedures and/or workplace requirements include the latest version of one or more of:

Australian and international standards covering the requirements for the competence of testing and calibration laboratories; laboratory safety; quality management; volumetric glassware; chemical analysis using techniques, such as ultraviolet-visible (UV-VIS) spectrophotometry or atomic absorption spectrometry

national work health and safety (WHS)standards and codes of practice, and national measurement regulations and guidelines

specific codes, guidelines, procedures and methods, such as the Australian code of good manufacturing practice for medicinal products (GMP), principles of good laboratory practice (GLP), Royal Australian Chemical Institute (RACI) and/or American Association of Cereal Chemists (AACC) methods for inorganic constituents

workplace documents, such as standard operating procedures (SOPs); quality and equipment manuals; calibration and maintenance schedules; material safety data sheets (MSDS) and safety procedures; material, production and product specifications; production and laboratory schedules; workplace recording and reporting procedures; and waste minimisation and safe disposal procedures

Techniques for preparation of samples

Techniques for preparation of samples include, but are not limited to, one or more of:

dissection, such as preparation of thymus extracts from mice

extraction (e.g. solvent extraction)

filtration (e.g. filter water samples and plate the sediment onto agar plates for incubation and growth of E. coli)

separation (e.g. dialysis)

precipitation and flocculation

centrifugation (excluding ultra centrifugation)

chromatography, such as:

gel filtration chromatography (e.g. crude purification of proteins)

affinity chromatography (e.g. purification of immunoglobulins)

electrophoresis, such as:

polyacrylamide gel electrophoresis for separation of DNA segments

agarose gel electrophoresis

capillary electrophoresis

gradient gel electrophoresis

Techniques to classify cells or species

Techniques to classify cells or species include, but are not limited to, one or more of:

classification of species according to taxa

classification of cells according to microscopic or staining characteristics

characteristics of bacterial colonies:

growth on differential media

colony morphology (size and shape)

biochemical reactions, such as miniaturised test strips, redox reactions and sugar tests

Techniques to analyse chemical and biological characteristics

Techniques to analyse chemical and biological characteristics include, but are not limited to, one or more of:

staining, such as:

Gram stain for gram negative and positive bacteria

Romanowsky stain for blood films

Haematoxylin and Eosin for tissue sections

Oil red O for fatty cellular inclusions

spore staining

flagella staining

microscopic examination, such as:

light

phase contrast

bright field

dark ground

enumeration

colorimetry and spectrophotometry, such as:

UV-VIS

fluorimetric

infrared

flame emission

atomic absorption spectrometry

electrochemistry, such as:

pH

ion selective electrodes and polarography (e.g. concentration of chloride ions)

chromatography, such as:

column and thin layer analytical and preparative chromatography

gas and liquid chromatography for purity, raw material and formulation checks

Safe work practices

Safe work practices include, but are not limited to, one or more of:

ensuring access to service shut-off points

recognising and observing hazard warnings and safety signs

labelling of samples, reagents, aliquoted samples and hazardous materials

handling and storage of hazardous materials and equipment in accordance with labelling, MSDS and manufacturer instructions

identifying and reporting operating problems or equipment malfunctions

cleaning and decontaminating equipment and work areas regularly using workplace procedures

using PPE, such as gloves, safety glasses, coveralls and gowns

using containment facilities (e.g. PCII, PCIII and PCIV physical containment laboratories), containment equipment (e.g. biohazard containers, laminar flow cabinets, Class I, II and III biohazard cabinets) and containment procedures

following established manual handling procedures

reporting abnormal emissions, discharges and airborne contaminants, such as noise, light, solids, liquids, water/waste water, gases, smoke, vapour, fumes, odour and particulates, to appropriate personnel

WHS and environmental management requirements

WHS and environmental management requirements include:

· complying with WHS and environmental management requirements at all times, which may be imposed through state/territory or federal legislation. These requirements must not be compromised at any time

· applying standard precautions relating to the potentially hazardous nature of samples

· accessing and applying current industry understanding of infection control issued by the National Health and Medical Research Council (NHMRC) and State and Territory Departments of Health, where relevant